Auxin-induced Changes in Avena Coleoptile Cell Wall Composition
نویسندگان
چکیده
منابع مشابه
Auxin-induced Changes in Avena Coleoptile Cell Wall Composition.
Sugar and uronic acid residues were derived from wall polysaccharides of oat (Avena sativa, var. Victory) coleoptiles by means of 2 N trifluoroacetic acid, 72% sulfuric acid, or enzymic hydrolysis. The products of hydrolysis were reduced and acetylated to form alditol acetates which were analyzed using gas chromatography. Time-course studies of auxin-promoted changes in various wall fractions i...
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The average molecular weight of the water soluble hemicelluloses (hemicellulose B) of the Avena coleoptile cell wall was determined by gel permeation chromatography (GPC) and viscometry. Analysis of the neutral sugar composition of henucellulose B eluted from a GPC column (Sepharose 4B) indicated that it consists of /J-glucan with a high molecular weight and arabinoxylan with a low molecular we...
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Sum rv01,1f. A mo(lifie(l )rocedlure is presente(d for the bioassay of aulxin lusilng -Iv'eno coleoptile segments. The modifications introduicedl restult in a substantial improvement of the commonly uised( coleoptile elongatioil tests. The proposed test retains the simplicity of physical reqillremeints and of manipulatioIn characteristic of all elongatioln tests. The maiin featuire of the propo...
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Several cell wall-bound glycosidases present in Avena sativa coleoptiles were assayed by following the hydrolysis of p-nitrophenyl-glycosides. Particular emphasis was placed on characterizing some parameters affecting the activity of beta-galactosidase. The pH optimum of this enzyme is 4.5 to 5.5; it is sensitive to copper ions and p-chloromercuribenzoate treatment and apparently has an excepti...
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A study has been made of the effects on hydroxyproline formation of 4 factors that influence the rate of cell elongation in the Avena coleoptile; auxin, sugars, an external osmoticum, and actinomycin D. Hydroxyproline formation is increased by a combination of auxin and sucrose, but is affected to a much lesser extent by either factor alone. Its formation is inhibited by an external osmoticum b...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1972
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.50.5.556